Composite

Part:BBa_K1878006

Designed by: Margaret Lie   Group: iGEM16_Rice   (2016-10-14)


mNarK/iRFP670


Usage and Biology

Hypoxia inducible iRFP670 with modified NarK promoter from 2009 UCL London (BBa_K239006)

The original citations are as follows:

  1. Kolesnikow, T., I. Schroder, and R.P. Gunsalus. "Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum." J Bacteriol, 1992. 174(22): p. 7104-11.
  2. Unden, G. and M. Trageser. "Oxygen regulated gene expression in Escherichia coli: control of anaerobic respiration by the FNR protein." Antonie Van Leeuwenhoek, 1991. 59(2): p. 65-76.
  3. Spiro, S. and J.R. Guest. "FNR and its role in oxygen-regulated gene expression in Escherichia coli." FEMS Microbiol Rev, 1990. 6(4): p. 399-428.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 645
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 731
    Illegal BsaI.rc site found at 288


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Categories
Parameters
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